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. 2022 Dec 15;17(12):e0278910. doi: 10.1371/journal.pone.0278910

Fig 1. LPS induces Glut1 expression in macrophages.

Fig 1

(a–c) RAW264.7 cells were treated with LPS (100 ng/mL) for 4 h, total RNA was isolated, reverse transcribed to cDNA, and RT-qPCR analyzed the expression Glut1. GAPDH was used as a control. The expression (relative to GAPDH) of Glut1 mRNA is shown in panel (a). Protein was extracted from macrophages and analyzed by Western blot using antibodies against Glut1 and GAPDH (loading control) (b). Quantifications (using ImageJ software) are shown in panel (c). Each experiment was repeated at least with three parallel replicates. Data represent mean ± SD (n = 3); **p < 0.001, ***p < 0.0001.