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. 2022 Dec 15;17(12):e0272177. doi: 10.1371/journal.pone.0272177

Fig 2. Imp RNAi and Imp overexpression result in reduced or increased Imp protein levels.

Fig 2

Wild type Imp levels are shown in Fig 1. (A) Imp RNAi within type II neuroblasts (inset: pnt-gal4 UAS-GFP) leads to lower Imp levels without disrupting the protein gradient; quantified in C. Scale bar, 20 μm. (C) Imp overexpression within type II neuroblasts (inset: pnt-gal4 UAS-GFP) leads to higher Imp levels without disrupting the protein gradient; quantified in C. Scale bar, 20 μm. (C, D) Quantification of Imp protein levels in type II neuroblasts in wild type, Imp RNAi, and Imp overexpression. (C) Histogram showing all n’s; (D) graph showing average values. n = 5 brains, each data point is one type II neuroblast. (E) Summary.