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. 2022 Dec 15;17(12):e0278895. doi: 10.1371/journal.pone.0278895

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© 2022 Summer et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — Spherical aggregates generated by cultivating 5000 MSCs on the lid of a petri dish using the hanging drop technology (A). Representative image of a spherical MSC aggregate adherent to a coverslip obtained by scanning electron microscopy (B) and image of a spherical MSC aggregate stained with a mAb specific for paxillin to reveal focal adhesions (green), with phalloidin to stain actin filaments (red), and DAPI to highlight the nuclei (blue) generated by laser scanning microscopy (C, D). Image of a spherical MSC aggregate embedded in HPL matrices showing sprout formation after 14 days of cultivation. The actin filaments were stained with phalloidin (green) and nuclei with DAPI (blue) (E, F).