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pH dependence of BAL-ODN immobilization to SC-glass slide. Radiolabeled probe 1 was diluted in three buffers of different pH ranging from 8.0 to 5.0 and deposited on the slide as 0.5 µl droplets. After incubation, blocking and washing the immobilized probe was quantified by phosphorimaging. The average spot size had a diameter of 1.5 mm. Values are presented as phosphorimager units per spot (an average of four spots).
