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Kinetics of BAL-ODN immobilization. An SC-glass slide was incubated in a humidified chamber at 37°C. A 100 mM solution of 32P-labeled probe 1 in 100 mM NaOAc, pH 5.0 was applied to the slide at different time points during the 4 h incubation period. The slide was blocked, washed and the amount of bound probe was determined by phosphorimaging. The average spot size had a diameter of 1.5mm. Attachment (fmol/spot) is plotted versus time. Values presented are means ± SD from four spots.
