Figure 6.

Concentration dependence of BAL-ODN probe density and effect on hybridization performance. A dilution series of ³²P-labeled or unlabeled probe 1 in 100 mM NaOAc, pH 5.0 was immobilized on two separate SC-glass slides for 3 h at 37°C. Slides were blocked and washed and phosphorimaged. The slide containing labeled probe was quantified to determine immobilization efficiency (open circles). The second slide was hybridized overnight at 37°C with 1 µM ³²P-labeled target 1 in 5× SSPE containing 0.1% Triton X-100. After extensive room temperature washing in hybridization buffer the slides were quantified to determine the amount of hybridized target ODN (closed circles). Values presented are means ± SD from four spots.