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. 2000 Jun;68(6):3129–3139. doi: 10.1128/iai.68.6.3129-3139.2000

FIG. 5.

FIG. 5

Expression product of the allelic FasA proteins by different plasmids. (A) Amino acid sequences of the amino terminus of the allelic FasA proteins. pDS167, pCS151, and pCS152 encode the genes for the wild-type 987P fimbriae; pRS234 encodes the genes for expressing chimeric fimbriae with the FasA-TGEV C protein (epitopes in boldface); and pCS101, pCS114, pCS154, and pCS155 encode the genes for expressing chimeric fimbriae with the FasA-TGEV C-TGEV A proteins (epitopes in boldface and italic, respectively). (B) SDS-PAGE of isolated wild-type or chimeric 987P fimbriae from different E. coli or serovar Typhimurium strains. Lane 1, protein molecular mass standards in kilodaltons; lane 2, E. coli SE5000/pDMS167; lane 3, E. coli SE5000/pRS234; lane 4, E. coli BL21/pCS101; lane 5, serovar Typhimurium SL3261/pCS101; lane 6, E. coli SE5000/pCS101; lane 7, E. coli SG396/pCS101. SE5000 (wild type for protease production) cleaved the chimeric FasA protein; BL21 (lon ompT), SG396 (htpR lonR9), and SL3261 did not cleave the chimeric FasA protein.