TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| E. coli | ||
| 987 | O9:K103:987P (prototype F6) | 35, 50 |
| SE5000 | MC4100 recA56 (Fim−) | 67 |
| χ6212 | ΔasdA1 derivative of DH5α | 52 |
| BL21 | lon ompT | Novagen, Madison, Wis. |
| SG396 | htpR lonR9 | 29 |
| Serovar Typhimurium | ||
| χ4487 | Δ(crp-cysG)-10 Δ(zhc-1431::Tn10) Δasd1 Δ(zhf-4::Tn10) Δcya-12 Δ(zid-62::Tn10) | R. Curtiss |
| χ4550 | gyrA1816 ΔasdA1 Δ(zhf-4::Tn10) Δcrp-1 Δcya-1 | 66 |
| SL3261 | ΔaroA | B. Stocker |
| CS3263 | ΔaroA ΔasdA1 | This study |
| Plasmids | ||
| pYA3332 | asd-containing vector | R. Curtiss |
| pLG339 | pSC101 derivative, Kmr | 70 |
| pDMS167 | pBR322 fas+ | 64 |
| pDMS203 | pDS167 fasD (Fas−) | 24, 64 |
| pRS234 | pDMS167 with TGEV C epitope in fasA (Fas+) | 56 |
| pCS101 | pRS234 with the TGEV A epitope in fasA (Fas+) | This study |
| pCS110 | pCS101 with PacI site changed to BamHI site (Fas+) | This study |
| pCS112 | pCS110 with AflII site changed to BamHI site (Fas+) | This study |
| pCS114 | pCS101 with asd gene in BamHI site (Fas+) | This study |
| pCS150 | pLG339 with the TGEV C and A epitopes inserted into fasA of pCS112 (Fas+) | This study |
| pCS151 | pDMS167 with asd in its BamHI site (Fas+) | This study |
| pCS152 | pDMS203 with asd in its BamHI site (Fas−) | This study |
| pCS154 | pCS150 asd (Fas+) | This study |
| pCS155 | pCS154 with the nirB promoter upstream fasA (Fas+) | This study |