Primers for cloning pCS2 +8 His6-Halo-•-hKeap1-(2×HA)	Primers for gene amplification (His6-Halo-•-hKeap1)*template: pFN21a Halo-TEV-Keap1	Fwd 1:CATGGGCAGCAGCCATCATCATCATCATCATGGGTCAGGGATGGCAGAAATCGGTACTGGRev 1:CCAGCGTAATCTGGAACATCGTATGGGTAGCTGCCACAGGTACAGTTCTGCTGGTCAATC
	Extension primers 1*template: PCR product from the above amplification step	Fwd ext 1:AGGTGACACTATAGAATACAAGCTACTTGTTCTTTTCCACCATGGGCAGCAGCCATCATCRev ext 1:AGCGTAATCTGGAACATCGTATGGGTAGCTGCCAGCGTAATCTGGAACATCGTATG
	Extension primers 2*template: PCR product from the above extension step*PCR product was inserted into pCS2 +8 empty vector	Fwd ext 2:GTCGGAGCAAGCTTGATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTCCACCRev ext 2:CGGCCTTTAATTAATGGCGCGCCACTAGTTTAAGCGTAATCTGGAACATCG
Primers for cloning pCS2 +8 His6-Halo-(2×HA)-P2A-•-hKeap1-(2×HA)	Primers for gene amplification (Halo) *template: pFN21a Halo-TEV-Keap1	Fwd 1: CATGGGCAGCAGCCATCATCATCATCATCATGGGTCAGGGATGGCAGAAATCGGTACTGG Rev 1: ATGGGTAGCTGCCGGCGTAGTCTGGGACGTCGTAAGGATAGCCGGAAATCTCGAGCGTCG
	Primers for gene amplification (hKeap1) *template: pFN21a Halo-TEV-Keap1	Fwd 1’: GCTGGAGACGTGGAGGAGAACCCTGGACCTGGCAGCGAGCCAACCACTGAGGATCTGTAC Rev 1’: CCAGCGTAATCTGGAACATCGTATGGGTAGCTGCCACAGGTACAGTTCTGCTGGTCAATC
	Extension primers 1 (Halo) *template: PCR product from the above amplification (Halo) step	Fwd ext 1: AGGTGACACTATAGAATACAAGCTACTTGTTCTTTTCCACCATGGGCAGCAGCCATCATC Rev ext 1: AAGTTAGTAGCTCCGCTTCCGGCGTAATCTGGAACATCGTATGGGTAGCTGCCGGCGTAG
	Extension primers 1’ (hKeap1) *template: PCR product from the above amplification (hKeap1) step	Fwd ext 1’: CGCCGGAAGCGGAGCTACTAACTTCAGCCTGCTGAAGCAGGCTGGAGACGTGGAGGAGAA Rev ext 1’: AGCGTAATCTGGAACATCGTATGGGTAGCTGCCAGCGTAATCTGGAACATCGTATG
	Extension primers 2 *template: PCR product from the above extension (hKeap1) step *Additional PCR with the two extended product (Halo and hKeap1) was done to yield a megaprimer to be inserted into pCS2 +8 empty vector	Fwd ext 2: GTCGGAGCAAGCTTGATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTCCACC Rev ext 2: CGGCCTTTAATTAATGGCGCGCCACTAGTTTAAGCGTAATCTGGAACATCG
Primers for cloning pCS2 +8 zKeap1a	Primers for gene amplification (zKeap1a) *template: zKeap1a cDNA	Fwd: ATTAAAGGCCGGCCAGCGATCGCCGGACCCACC ATGATATGTCCAAGAAAGAAGAGGC Rev: TCTAGAGGCTCGAGAGGCCTTGAATTCGATCACATCTCCTCGTCCACTTC
	Extension primers *template: PCR product from the above amplification step *PCR product was inserted into pCS2 +8 empty vector	Fwd: GCTACTTGTTCTTTTTGCAGGATCCACTAGTGGCGCGCCATTAATTAAAGGCCGGCCAGC Rev: CTTATCATGTCTGGATCTACGTAATACGACTCACTATAGTTCTAGAGGCTCGAGAGGCCT
Primers for cloning pCS2 +8 myc-Halo-•–3×Flag-zKeap1a	Primers for gene amplification (myc-Halo-•–3×Flag) *template (first PCR with Fwd and Rev): pCS2 +8 myc-Halo-TEV-3x Flag *template (second PCR with Fwd and Rev extender): PCR product from first reaction *PCR product was inserted into pCS2 +8 zKeap1a	Fwd: GCTACTTGTTCTTTTTGCAGGATCCACTAGTGGCGCGCCATTAATTAAAGGCCGGCCAGC Rev: GCCTCTTCTTTCTTGGACATATCATTTTATCATCATCATCTTTATAATCAATATCATGAT Rev extender: ACGATGGCGGAGAAATCCTCATCTTTGATGGGCCTCTTCTTTCTTGGACATATCAT
Primers for introducing synonymous mutations in pCS2 +8 myc-Halo-•–3×Flag-zKeap1a, preventing zKeap1a-ATG-MO binding	Primers for gene amplification *template (first PCR with Fwd and Rev): pCS2 +8 myc-Halo-•–3xFlag-zKeap1a *template (second PCR with Fwd and Rev extender): PCR product from first reaction *PCR product was inserted into pCS2 +8 myc-Halo-•–3xFlag-zKeap1a	Fwd: CAGAAATCGGTACTGGCTTTCCA Rev: GCGTTTCTTGCGCGGGCAGATCATTTTATCATCATCATCTTTATAATCAATATCATGATC Rev ext: GTCGGAGCAAGCTTGATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTCCACC
Primers for cloning pCS2 +8 zKeap1b	Primers for gene amplification (zKeap1b) *template: zKeap1b cDNA	Fwd: ATTAAAGGCCGGCCAGCGATCGCCGGACCCACC ATGTTGGCGGCGGC Rev: TCTAGAGGCTCGAGAGGCCTTGAATTCGAGCACTGACAGGGGATCAGC
	Extension primers *template: PCR product from the above amplification step *PCR product was inserted into pCS2 +8 empty vector	Fwd: GCTACTTGTTCTTTTTGCAGGATCCACTAGTGGCGCGCCATTAATTAAAGGCCGGCCAGC Rev: CTTATCATGTCTGGATCTACGTAATACGACTCACTATAGTTCTAGAGGCTCGAGAGGCCT
	Stop codon mutation primers (site-directed mutagenesis) *template: plasmid obtained from the above gene insertion step	Fwd: CACAAAGAGCTGATCCCCTGTCAGTGCTAATCGAATTCAAGGCCTCTCGAGCCTCTAGA Rev: TCTAGAGGCTCGAGAGGCCTTGAATTCGATTAGCACTGACAGGGGATCAGCTCTTTGTG
Primers for cloning pCS2 +8 myc-Halo-•–3×Flag-zKeap1b	Primers for gene amplification (myc-Halo-•–3×Flag) *template (first PCR with Fwd and Rev): pCS2 +8 myc-Halo-TEV-3x Flag *template (second PCR with Fwd and Rev extender): PCR product from first reaction *PCR product was inserted into pCS2 +8 zKeap1b	Fwd: GCTACTTGTTCTTTTTGCAGGATCCACTAGTGGCGCGCCATTAATTAAAGGCCGGCCAGC Rev: CGTCATGCCCGCCGCCGCCAACATTTTATCATCATCATCTTTATAATCAATATCATGAT Rev ext: CCATTGCTGGCCGACGGCGTCACCTCCGCCTTACA CTCCGTCATGCCCGC