| Strain, strain background (Escherichia coli) |
XL10-Gold Ultracompetent Cells |
Agilent Technologies |
Cat# 200315 |
N/A |
| Cell line (Homo-sapiens) |
293T (ATCC CRL-3216) |
ATCC |
Cat# CRL-3216, RRID:CVCL_0063
|
https://www.atcc.org/products/crl-3216
|
| Genetic reagent (Danio rerio) |
Tg(–3.5gstp1:GFP)/it416b
|
Japanese National BioResource Project |
N/A |
https://shigen.nig.ac.jp/zebra/index_en.html
|
| Genetic reagent (Danio rerio) |
Brian’s wildtype |
Professor Joseph Fetcho’s lab (Cornell University) |
N/A |
A strain of wt-zebrafish that shows low pigmentation |
| Genetic reagent (Danio rerio) |
Wild-type line Casper
|
Professor Joseph Fetcho’s lab (Cornell University) |
PMCID:PMC2292119
|
N/A |
| Recombinant DNA reagent |
pGL4.37[luc2P/ARE/Hygro] |
Promega |
Cat# E3641 |
N/A |
| Recombinant DNA reagent |
pGL4.75[hRluc/CMV] |
Promega |
Cat# E6931 |
N/A |
| Recombinant DNA reagent |
pCDNA3 myc-Nrf2 |
Addgene |
Cat# 21555, RRID:Addgene_21555
|
N/A |
| Recombinant DNA reagent |
pCS2 +8 vector |
Addgene |
Cat# 34931, RRID:Addgene_34931
|
N/A |
| Recombinant DNA reagent |
pFN21a Halo-TEV-Keap1 |
Halo ORFeome, Promega Kazusa collection |
Cat# FHC00420 |
N/A |
| Antibody |
Anti-GFP (FITC) (goat polyclonal) |
Abcam |
Cat# ab6662, RRID: AB_305635
|
IF (1:1000) |
| Antibody |
Anti-Flag (mouse monoclonal) |
Sigma |
Cat# F3165, RRID:AB_259529
|
WB (1:4000) |
| Antibody |
Anti-Keap1 (mouse monoclonal) |
Abcam |
Cat# ab119403, RRID: AB_10903761
|
IF (1:500) |
| Antibody |
Anti-HaloTag (rabbit polyclonal) |
Promega |
Cat# G9281, RRID:AB_713650
|
WB (1:3000) |
| Antibody |
Anti-HA HRP (mouse monoclonal) |
Sigma |
Cat# H3663, RRID: AB_262051
|
IF and WB (1:500) |
| Antibody |
Anti-β-actin HRP (mouse monoclonal) |
Sigma |
Cat# A3854, RRID: AB_262011
|
WB (1:30,000) |
| Antibody |
Anti-mouse-HRP (horse polyclonal) |
Cell Signaling |
Cat# 7076, RRID:AB_330924
|
WB (1:2000) |
| Antibody |
Anti-rabbit-HRP (goat polyclonal) |
Cell Signaling |
Cat# 7074, RRID:AB_2099233
|
WB (1:2000) |
| Antibody |
Anti-goat IgG H&L (AlexaFluor568) (rabbit polyclonal) |
Abcam |
Cat# ab175707, RRID: AB_2923275
|
IF (1:2000) |
| Antibody |
Anti-rat IgG H&L (AlexaFluor568) (donkey polyclonal) |
Abcam |
Cat# ab175475, RRID: AB_2636887
|
IF (1:500) |
| Antibody |
Anti-mouse IgG H&L (AlexaFluor568) (donkey polyclonal) |
Abcam |
Cat# ab175472, RRID: AB_2636996
|
IF (1:1000) |
| Antibody |
Anti-mouse IgG H&L (AlexaFluor647) (donkey polyclonal) |
Abcam |
Cat# ab150107, RRID:AB_2890037
|
IF (1:1000) |
