Figure 7.
CLP surgery was performed on mice. Mice were given vehicle saline or RvD2 48h after surgery. 24h after injections, mice were given P. aeruginosa intranasally. 24h after the 2nd hit of p. aeruginosa, mice were sacrificed and lungs lavaged. Using flow cytometry, cytokines in lungs including TNF-α, IL-6, IL-10, IFN-γ, IFN-β, IL-1β, IL-17, IL-23, and MCP-1 were quantified by bead-based immunoassay. RvD2 significantly reduced lung lavage fluid IL-23 levels. Data are mean ± S.E.M. ** P < 0.01 for n = 10 – 12 in all groups.