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. 2022 Sep 30;21:100495. doi: 10.1016/j.ynstr.2022.100495

Fig. 6.

Fig. 6

Reverse correlation between Atf3 and IFN-regulated genes after chronic stress. (A–D) IFN levels in (A–C) spleen (N = 13–15/group) and (D) brain (N = 4–11/group) from the stressed (ST) and control (CO) mice. Unpaired t-test. Mean ± SEM. (E) IGV map track shows RNA-seq signal at Atf3 gene locus. Notice increased signal in ST. (F) Real-time RT-PCR validation for Atf3. N = 3/group. Mean ± SEM. Unpaired t-test, one-tailed, *P < 0.05. (G) Overlap between the top 50 genes up-regulated in Atf3 knockout macrophage (GSE61055) and genes down-regulated in the microglia after chronic stress. (H) Heatmap shows the expression of 20 overlapping genes in panel G. (I) GO analysis for the 20 overlapping genes. Notice the top enrichment for IFN-β pathways. (J) List of ATF3 binding motifs at the promoters (TSS ± 5 kb) from 11 of the 20 overlapping genes.