Fig. 5. Chemogenetic silencing of STN neurons reduces the response of LPBGlu neurons to peripheral mechanical stimulation.
a Experimental approach for GCaMP6 signal recordings of LPBGlu neurons with chemogenetic inhibition of STN neurons. b Example images of hM4Di and GCaMP6f expression in the injection sites. c–j Heat maps (c, g), example traces (d, h), average traces (e, i), and quantification (f, j, AUC in panels e and i) of GCaMP6f signal in the LPB of mice receiving von Frey stimulation (4 g) of hind paws with i.p. injection of saline or CNO (3 mg/kg). f F(1,8) = 7.69, p = 0.02, n = 5 mice. j F(1,8) = 1.06, p = 0.33; n = 5 mice. CNO or the same volume of saline was applied 45 min prior to GCaMP6f signal recording. *P < 0.05, **P < 0.01; Two-way ANOVA with Tukey’s post-hoc analysis for (f and j). Data are presented as mean ± SEM. AU in panels (d) and (h) stands for arbitrary unit of fluorescence intensity. Scale bars: 100 µm.