Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Dec 15;13:7766. doi: 10.1038/s41467-022-35303-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — A Skeletal preparation of E18.5 embryos, dorsal view, revealed an increase in the number of thoraco-lumbar elements in Cdx2P:Nr6a1 embryos (n = 2 independent samples) compared to wildtype (n = 8 independent samples). C = cervical, T = thoracic, L = lumbar, S = sacral and Cd = caudal vertebrae. B Higher magnification, lateral view, of the lumbar region in embryos from (A) revealed the anapophysis (red arrow) normally present on the first 3 lumbar elements in wildtype was now present on 7 of the 8 lumbar elements in Cdx2P:Nr6a1 embryos. Experimental numbers as per 5A. C RNAseq analysis of E10.5 wildtype and Cdx2P:Nr6a1 tailbuds revealed widespread changes in Hox expression downstream of Nr6a1. Results are presented as a log2-transformed fold change in Cdx2P:Nr6a1 samples relative to wildtype, n = 2 for Cdx2P:Nr6a1 and n = 4 for wildtype. Only those Hox genes with a false discovery rate (FDR) < 0.05 are displayed, and are colour-coded based on the axial region where the Hox protein functions. Source data are provided as a Source Data file. D Whole mount in situ hybridisation revealed reduced expression of Hoxd12 within the tail of E10.5 Cdx2P:Nr6a1embryos compared to wildtype. Consistent spatial changes were observed in 2 biologically independent samples/genotype. E Venn diagram overlay of differentially expressed genes (DEGs) in Cdx2P-Nr6a1 (this work) and Gdf11^−/− ²⁸ tailbud tissue reveals substantial overlap. Source data are provided as a Source Data file. F Analysis of the 151 co-regulated genes identified in (D), presented here as log2-transformed fold change in Cdx2P:Nr6a1 samples relative to wildtype, revealed that 136 genes (90%) displayed the same direction of regulation in both genetically altered backgrounds. Red and blue dots indicate upregulated and downregulated genes in Gdf11^−/− tailbuds, respectively. Source data are provided as a Source Data file.