Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Dec 15;13:7766. doi: 10.1038/s41467-022-35303-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 8 — A Heightened neural (red) and reduced mesodermal (blue) gene expression signatures were observed following prolonged maintenance of Nr6a1 within the mouse tailbud. RNAseq analysis was presented as a log2-transformed fold change in Cdx2P:Nr6a1 samples relative to wildtype, n = 2 for Cdx2P:Nr6a1 and n = 4 for wildtype. Neural and mesodermal gene lists were selected based on in vivo single-cell RNAseq analysis²⁰, and only genes with a false discovery rate (FDR) < 0.05 are displayed. Source data are provided as a Source Data file. B Heightened mesodermal (blue) and reduced neural (red) gene expression signatures were observed in Nr6a1^−/− in vitro-derived NMPs compared to wildtype. In vitro differentiation protocol schematised, analysis time-point (d3) marked with asterisk. RNAseq analysis is presented as a log2-transformed fold change in Nr6a1^−/− samples relative to wildtype, n = 3/genotype. Neural and mesodermal gene lists as per above, and only genes with a false discovery rate (FDR) < 0.05 are displayed. Source data are provided as a Source Data file. C Flow cytometry analysis of Sox2 and T/Bra protein expression within cells of the E9.0 tailbud, collected from WT (n = 4 biologically independent samples) and TCre^ERT2;Nr6a1^flx/flx (n = 9 biologically independent samples) embryos. Single Sox2-positive, single T/Bra-positive, or dual positive cells are presented as a percentage of all single, viable cells. Statistical comparison between genotypes was performed using an unpaired t-test with Welch’s correction, p-values are provided in the figure.