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. 2022 Dec 15;13:7778. doi: 10.1038/s41467-022-35503-y

Fig. 5. In vitro therapeutic mechanism exploration of cardiac protection.

Fig. 5

a KEGG enrichment pathway for DOX-treated cells as compared to the untreated cells. Significant tests (padj) are based on the negative binomial distribution with further corrections (Benjamini–Hochberg procedure). *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001. b Distributions of the cell-apoptosis-associated genes in different groups. n = 3. Data are presented as mean ± s.d. c and d Protein expressions of Cycs, Bax, Bcl-xl (c), cleaved-Parp1, Aif and cleaved-caspase-3 (d) within cells treated in different groups. e and f Confocal microscopic images of DCFH-DA-stained (e) or FerroOrange-stained (f) H9c2 cells received several treatments indicated. Scale bar: 50 μm. g Schematic illustrations of the intracellular iron overload, which will lead to oxidative stress and cell apoptosis. h Distributions of the iron-transport-associated genes in different groups. n = 3. Data are presented as mean ± s.d. i Protein expressions for Ho-1, Slc40a1, Tfrc, Nrf2 and Fth1 within cells treated in different groups.