TABLE 2.
Homolog and expression level identification of the top 10 isobutanol producers from Figure 2 via Sanger and Nanopore sequencing. Green, yellow, and red boxes represent a strong, medium, or weak strength promoter, respectively (See Supplementary Data Sheet S1 for Uniprot IDs).
| ALS | KARI | DHAD | KDC | ADH | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Cassette | Promoter | Homolog | Promoter | Homolog | Promoter | Homolog | Promoter | Homolog | Promoter | Homolog |
| #1 | P SAC6 | Trichoderma gamsii | P HHF2 | Olsenella scatoligenes | P RET2 | Hypoxylon sp. | P POP6 | Dermatophilus congolensis | P PAB1 | Lactococcus lactis |
| #2 | P SAC6 | Trichoderma gamsii | P HHF2 | Lachnospiraceae bacterium | P RET2 | Jeotgalibaca sp. | P ALD6 | Frondihabitans sp. 762G35 | P PAB1 | Gluconacetobacter diazotrophicus |
| #3 | P SAC6 | Talaromyces stipitatus | P RNR2 | Brevundimonas vesicularis | P RPL18B | Saccharomonospora marina | P TEF1 | Enterococcus rotai | P PAB1 | Acetobacter indonesiensis |
| #4 | P HTB2 | Brevibacterium linens | P RNR2 | Streptomyces griseorubiginosus | P RPL18B | Acidimicrobiaceae bacterium | P TEF1 | Helicobacter ailurogastricus | P TEF2 | n.a. |
| #5 | P SAC6 | Bifidobacterium mongoliense | P RNR2 | Slackia exigua | P RPL18B | Arthrobacter alpinus | P TEF1 | Enterococcus rotai | P RNR1 | Tanticharoenia sakaeratensis |
| #6 | P SAC6 | Thiohalomonas denitrificans | P HHF2 | Lactonifactor longoviformis DSM 17459 | P RPL18B | Penicillium arizonense | P TEF1 | Helicobacter ailurogastricus | P PAB1 | Arthrobacter sp. RC1.1 241 |
| #7 | P SAC6 | Oidiodendron maius | P RNR2 | Shewanella sp. | P RPL18B | Lactococcus piscium | P TEF1 | Enterococcus rotai | P RNR1 | Tanticharoenia sakaeratensis |
| #8 | P SAC6 | Streptomyces viridochromogenes | P RNR2 | Shewanella sp. | P RPL18B | Methanobrevibacter smithii | P TEF1 | Helicobacter ailurogastricus | P PAB1 | Chlamydia trachomatis |
| #9 | P SAC6 | Aspergillus nomius | P HHF1 | Clostridium populeti | P THD3 | Methanosphaera stadtmanae | P TEF1 | Enterococcus rotai | P PAB1 | Lactococcus lactis subsp. lactis (strain IL1403) |
| #10 | P PGK1 | Talaromyces stipitatus (strain ATCC 10500) | P RNR2 | Alphaproteobacteria bacterium | P THD3 | Thiohalobacter thiocyanaticus | P TEF1 | Enterococcus rotai | P PAB1 | Arthrobacter sp. RC1.1 241 |
n.a, not available; sequencing reads could not distinguish the homolog.