TABLE 3.

Fermentation products of LbIlvC variants. Aerobic production of strains sJD189, sJD107, sFVG612, and sFVG613. Fermentations were performed in SCD-ura + 70 mM ethanol. Isobutanol, pyruvate and ethanol titers were measured after 3 days. Standard deviation was calculated from 3 biological replicates.

Strain	Plasmid	Isobutanol (mg/L)	Pyruvate (g/L)	Ethanol (g/L)
sJD189	pCC1FOSY	0 ± 0	1.6 ± 0.2	0.3 ± 0.3
sJD107	p#2 (WT LbIlvC)	283.6 ± 14.8	0.7 ± 0.1	0 ± 0
sFVG612	pFVG605 (LbIlvC DD)	69.3 ± 10	0.9 ± 0	0 ± 0
sFVG613	pFVG606 (LbIlvC DDV)	154.2 ± 11.5	1.6 ± 0.2	0 ± 0