Table 4.
Antibacterial activity of A. hydaspica: isolated fractions/compounds from AHE by flash chromatography (ISCO)
| Antibacterial activity | Microorganism | ||||||
|---|---|---|---|---|---|---|---|
| Samples | Gram + ive | Gram − ive | |||||
| S. aureus | E. faecalis | B. Subtilis | E. coli | P. aeruginosa | K. pneumoniae | A. baumannii | |
| % GI | % GI | % GI | % GI | % GI | % GI | % GI | |
| AHE/F3 | 91 ± 1.17*,+++ | 44 ± 1.23 | 90 ± 1.71*,+++ | 99 ± 0.99*,+++ | 46 ± 0.87 | 31 ± 0.98 | 25 ± 0.56 |
| AHE/F5 | 21 ± 0.67 | 18 ± 0.31 | 46 ± 1.01 | 62 ± 1.10+++ | 15 ± 0.16 | 8.0 ± 0.62 | 11 ± 0.13 |
| AHE/F7 | 24 ± 0.52 | 16 ± 0.27 | 70 ± 1.30+++ | 24 ± 0.18 | 1.0 ± 0.02 | 6.6 ± 0.25 | − 3 ± 0.05 |
| Tetracycline | 95 ± 1.21 | – | 99 ± 1.79 | 96 ± 1.43 | 94 ± 1.13 | 95 ± 1.11 | 96 ± 1.17 |
| Penicillin G | – | 94 ± 1.66 | – | – | – | – | – |
| Antifungal activity | Microorganism | |||
|---|---|---|---|---|
| Yeast | Fungi | |||
| Sample | C. neoformans | C. albicans | F. solani | A. niger |
| % GI | % GI | % GI | % GI | |
| AHE/F3 | 20.62 ± 0.34 | 30.3 ± 0.41 | 87.2 ± 0.94* | 86.3 ± 1.0* |
| Amphotericin B | 94.90 ± 1.05 | 82.70 ± 0.96 | 90.1 ± 1.01 | 92.4 ± 1.11 |
Values are expressed as mean ± SEM (n = 3). Significant activity = more than 70% growth inhibition; Good activity = 60–70% growth inhibition; Moderate activity = 50–60% growth inhibition; non-significant below 50% growth inhibition. Symbol (–) represents not done. GI (growth inhibition at 100 µg/ml)
*Indicate highest growth inhibitory potential (GI %) of fraction, and fraction with * activity was subjected for further testing to get MIC50 values and structure elucidation
+++Indicated significant (p < 0.0001) inhibitory potential of selected fraction compared to other fractions. Data analyzed by One-way ANOVA followed by Tukey’s comparison analysis