Table 4.
Summary of the downstream diagnostics from experimental total RNA extracts (N = 90).
| Muscle | N | 260/230 | 260/280 | Total RNA (ng) | RIN | 28S/18S | No. reads | Pseudo aligned reads | Mapping ratio |
|---|---|---|---|---|---|---|---|---|---|
| PCA | 29 | 1.03 ± 0.53 | 2.15 ± 0.09 | 1513.68 ± 860.68 | 7.8 ± 0.3 | 1.4 ± 0.2 | 4.19E+07 ± 7.23E+06 | 3.33E+07 ± 5.88E+06 | 79.4 ± 1.5 |
| LTA | 30 | 1.08 ± 0.47 | 2.12 ± 0.06 | 1436.82 ± 903.31 | 7.9 ± 0.4 | 1.4 ± 0.2 | 4.22E+07 ± 7.42E+06 | 3.31E+07 ± 5.83E+06 | 78.5 ± 1.8 |
| MTA | 29 | 1.10 ± 0.45 | 2.13 ± 0.06 | 1081.44 ± 397.08 | 8.1 ± 0.5 | 1.4 ± 0.2 | 4.04E+07 ± 6.82E+06 | 3.13E+07 ± 5.45E+06 | 77.5 ± 1.7 |
| Control | 2 | 0.85 ± 0.36 | 2.14 ± 0.19 | 413.55 ± 25.63 | 8.1 ± 0.7 | 1.6 ± 0.1 | 4.17E+07 ± 7.22E+06 | 3.37E+07 ± 5.99E+06 | 80.9 ± 0.3 |
RNA samples were sequenced using a standard RNA-Seq pipeline (Illumina NovaSeq 6000), and sequencing reads were quantified using Kallisto.
Note, the controls in this experiment were (1) RNA extracts from the right ILMs aggregated and (2) RNA extracts from all paired ILMs combined.