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. 2001 Dec 15;29(24):5058–5066. doi: 10.1093/nar/29.24.5058

Figure 3.

Figure 3

Figure 3

Inhibition of RepA ATPase activity by flavone derivatives. (A) Inhibitory effect of flavone compounds on RepA ATPase activity stimulated by ssDNA. Reactions were performed in buffer A as described in the Materials and Methods. ATPase activity is normalized to the activity in the absence of inhibitor. (B) Double-reciprocal Lineweaver–Burke plot describing the kinetic inhibition of ssDNA-dependent RepA ATPase activity by myricetin. The assays were carried out as described in the Materials and Methods except that myricetin was added in indicated amounts to reaction mixtures containing 100 or 200 µM of ATP.