Figure 4.

Dezocine inhibits the proliferation and migration of ovarian cancer by down-regulating CRABP2. (a) By RNA-Seq analysis, the DEGs (|log₂ fold change| > 1 & Q value < 0.001) in ES-2 cells treated with dezocine. (b) Expression of CRABP2 mRNA was detected by RT-PCR analysis in ES-2 and SKOV3 cells treated with dezocine. (c) Expression of CRABP2 protein was detected by western blot analysis in ES-2 and SKOV3 cells treated with dezocine or dezocine + pcDNA3.1-CRABP2. (d and e) ES-2 (d) and SKOV3 (e) cells were treated with dezocine or dezocine + pcDNA3.1-CRABP2, and cell viability was measured by CCK8 assay. (f and g) After indicated treatment, cell migration was measured by transwell assay. ^** P < 0.01.