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. 2022 Dec 2;10:1059083. doi: 10.3389/fped.2022.1059083

Figure 1.

Figure 1

Mice are more prone to EAE post-puberty in induced and T cell receptor transgenic models. (A) Puberty can be estimated in female mice by measuring vaginal opening (first sign), which is confirmed by first estrus or in male mice by measuring the age at pre-putial separation (when the prepuce can be easily separated from the glans penis). Shown is the mean + SEM age of pre-putial separation in male (N = 36), and vaginal opening and first estrus in female (N = 28) C57BL6/J mice. (B) Incidence of EAE observed in female and male SJL/J mice induced at 8 weeks of age with PLP p139-151/CFA that had received a pre-pubertal gonadectomy or sham surgery at the age of 4 weeks. Data shown represents the disease-free survival in mice over 30 days of observation. N = 14 for pre-pubertal females (pre-pubertal oophorectomy), N = 15 for post-pubertal female (pre-pubertal sham surgery), N = 11 for pre-pubertal male (pre-pubertal castration), N = 12 for post-pubertal male (pre-pubertal sham surgery). *Significant by Log-rank test (P < 0.05). (C,D) The number of cases of spontaneous EAE (C), and the incidence of paralysis (D) in MOG TCR transgenic mice over a period of 2 years.