Figure 1.

Neutrophil differentiation in steady state. (A) Traditional characterization of neutrophil development includes the following stages: GMP, myeloblast, promyelocyte, myelocyte, metamyelocyte, band and segmented neutrophil. (B) On the basis of trajectory analysis, the first 5 clusters, GMP, proNeu, preNeu, immNeu, and mNeu, originate primarily from the BM and represent neutrophil development in the BM. Neutrophil differentiation in BM is divided into 2 phases: cell division and post-mitotic maturation. The cell division stage is defined when GMP differentiate into proNeu and preNeu sequentially. The post-mitotic maturation stage encompasses immNeu, which give rise to non-proliferating mNeu. PMNa, PMNb, and PMNc are the major neutrophil subpopulations in the peripheral blood (PB) and spleen (SP). PMNa cells in the PB are derived from both mNeu and immNeu cells, whereas PMNb cells arise primarily from BM mNeu cells in the steady state.