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. 2022 Oct 12;82(24):4670–4679. doi: 10.1158/0008-5472.CAN-22-0787

Figure 3.

Figure 3. Effect of doxorubicin or Cdk4/6i on the bystander effect of SYD985. A, Schematic showing the experimental procedure. The indicated cells were treated with doxorubicin or Cdk4/6i for 4 or 5 days, respectively. Then, cells were harvested and cococultured with MDA-MB-231 cells expressing luciferase and cocultures were treated with SYD985 or T-DM1 for 5 additional days. At the end of the experiment, MDA-MB-231/luc cells were quantified by luminescence. B and C, Luminescence, quantified as described in A, was determined at the end of the experiments and normalized to cells treated with vehicle. n ≥ 3.

Effect of doxorubicin or Cdk4/6i on the bystander effect of SYD985. A, Schematic showing the experimental procedure. The indicated cells were treated with doxorubicin or Cdk4/6i for 4 or 5 days, respectively. Then, cells were harvested and cococultured with MDA-MB-231 cells expressing luciferase and cocultures were treated with SYD985 or T-DM1 for 5 additional days. At the end of the experiment, MDA-MB-231/luc cells were quantified by luminescence. B and C, Luminescence, quantified as described in A, was determined at the end of the experiments and normalized to cells treated with vehicle. n ≥ 3. ns, nonsignificant; *, P < 0.05; **, P < 0.01.