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. 2022 Oct 12;82(24):4670–4679. doi: 10.1158/0008-5472.CAN-22-0787

Figure 4.

Figure 4. Role of cathepsins on the bystander effect of SYD985. A, Levels of the indicated cathepsins were determined by qRT-PCR in MCF7 cells treated as in Fig. 1A; n ≥ 3. B, Levels of mRNA encoding CTSB in parental MCF7 cells or the same cells stably transfected with the empty vector or two different shRNA (sh) targeting CTSB. Results were normalized to parental cells; n = 3. C, As described in Fig. 3A, cocultures were performed to analyze the bystander effect of SYD985 upon knockdown of CTSB; n = 3. D and E, The role of Cathepsin L on the bystander effect of SYD985 was analyzed as in B and C. MCF7 cells treated as indicated were lysed and lysates analyzed by Western blot analysis with anti-CTSB antibodies (F and G) or processed to determine CTSB activity with a specific assay (H); n = 3.

Role of cathepsins on the bystander effect of SYD985. A, Levels of the indicated cathepsins were determined by qRT-PCR in MCF7 cells treated as in Fig. 1A. n ≥ 3. B, Levels of mRNA encoding CTSB in parental MCF7 cells or the same cells stably transfected with the empty vector or two different shRNA (sh) targeting CTSB. Results were normalized to parental cells. n = 3. C, As described in Fig. 3A, cocultures were performed to analyze the bystander effect of SYD985 upon knockdown of CTSB. n = 3. D and E, The role of cathepsin L on the bystander effect of SYD985 was analyzed as in B and C. F–H, MCF7 cells treated as indicated were lysed and lysates analyzed by Western blot analysis with anti-CTSB antibodies (F and G) or processed to determine CTSB activity with a specific assay (H). n = 3. *, P < 0.05; **, P < 0.01.