FIG. 1.

Production of an extracellular signaling molecule by H. pylori. V. harveyi BB170 was inoculated into AB medium containing 10% CM from V. harveyi BB152, 10% CM from H. pylori 26695, or 10% sterile brucella broth. Cultures were incubated at 25°C with constant agitation, and aliquots were removed at serial time points for measurement of luminescence (A) and viable cell count (B). Luminescence is expressed in relative light units (luminescence per 10⁶ viable V. harveyi BB170 cells). At the 5-h time point, CM from V. harveyi BB152 and H. pylori 26695 induced >100-fold-greater luminescence than did sterile brucella broth.