Figure 3.

Immune infiltration in different molecular subtypes of EC by multiplex immunofluorescence. (A) The immune infiltrations in EC of POLE mutant subtype. Intense red fluorescence indicates large amount of CD8⁺ cell infiltration. (B) The immune infiltration in EC of MSI-H subtype. (C) The immune infiltration in NSMP subtype. Few fluorescence signals could be observed, indicating absence of immune infiltration. (D) The immune infiltration in TP53 mutant subtype. Intense yellow fluorescence indicates the infiltration of FOXP3⁺ cells. For each subtype, a representative filed was selected, and the major tumor regions are outlined. TMB-H, high tumor mutation burden; MSI-H, microsatellite instability high; NSMP, no specific molecular profile; EC, endometrial cancer.