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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Nanomedicine. 2022 Oct 17;47:102614. doi: 10.1016/j.nano.2022.102614

Figure 1. Characterization of NP-N1 and NP-N2 nanoparticles.

Figure 1.

A. Diagram of layered NP-NA nanoparticle generation. NP protein nanoparticles (NP nano) were generated by ethanol desolvation. Double-layered NP-NA protein nanoparticles were formed by crosslinking NA proteins onto the surfaces of the NP particle cores. B, C. Coomassie blue staining and Western Blotting analysis of NP-NA nanoparticles with anti-6×His-tag antibodies. Soluble N1 and NP (sN1 and sNP) were used as controls for NP-N1 Western Blotting analysis. NP and N1 showed a similar migration in the SDS-PAGE gel. D. Size distribution of protein nanoparticles. E. Cytokines (IL-6 and TNF-α) secretion in JAWS II cells treated with sNP + sN1, NP-N1, and NP-N2 at a concentration of 5 μg/mL. (n = 3; **p < 0.01; ***p < 0.001; ns, p > 0.05)