Table 2.
Anisakid detection in artificially contaminated fish samples analyzed by RPA-SG and qPCR assay.
| Concentration*(%) | Without heating | Boiling for 15 min | ||
|---|---|---|---|---|
| RPA-SG | qPCR (Cq)# | RPA-SG | qPCR (Cq)# | |
| 0.0005 | − | 35.46 ± 0.22 | − | 36.73 ± 0.24 |
| 0.001 | − | 34.70 ± 0.19 | − | 35.90 ± 0.18 |
| 0.005 | + | 32.37 ± 0.17 | − | 33.55 ± 0.20 |
| 0.01 | + | 31.55 ± 0.15 | + | 32.14 ± 0.18 |
| 0.05 | + | 29.53 ± 0.18 | + | 30.73 ± 0.20 |
“+” = positive results, “–” = negative results. *Marine fish samples were spiked with different concentration of anisakid larvae (w/w). # The data are presented as mean ± standard deviation (SD). Each concentration had three triplicates.