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. 2022 Dec 16;9:70. doi: 10.1186/s40779-022-00429-5

Table 1.

Summary of typical cell viability studies

Method Bioink Application Cell viability References
Inkjet-based bioprinting PEGDMA, human chondrocytes Cartilage-like constructs 89% [139]
Sodium alginate, 3T3 fibroblasts Zigzag tubes 82% [140]
Sodium alginate, 3T3 fibroblasts Vascular-like constructs > 90% after 1 d [141]
PEG/peptide, hMSCs Cartilage-like constructs 89% [86]
Extrusion-based bioprinting Nanofibrillated cellulose, alginate, hNCs Aricular constructs 70% [44]
Alginate sulfate, nanocellulose, chondrocytes Miniature ears > 85% at day 14 [142]
Polyurethane solutions and NSCs Nerve-like constructs > 50% after 1 d [143]
Alginate, fibroblasts, smooth muscle cells Vascular-like constructs > 91% after 7 d [9]
Laser-assisted bioprinting Alginate, 3T3 fibroblasts 3D constructs > 90% [144]
HUVECs, biopaper Osseous constructs 92.4% [72]
3T3 fibroblasts 3D constructs > 93.5% [145]
Hyaluronic acid, hiPSCs 3D constructs > 94% with control effect [146]
Stereolithography-based bioprinting GelMA, PEGDA, EY, 3T3 fibroblasts 3D structure 85% for at least 5 d [21]
GelMA, EY, 3T3 fibroblasts 3D structure 91.5% [124]
GelMA, HUVECs, C3H/10T1/2 cells Prevascularized tissues > 85% [147]
GelMA, LAP, mBMSCs Native-like tissues 95% [148]

PEGDMA poly(ethylene glycol) dimethacrylate, PEG polyethylene glycol, hMSCs human mesenchymal stem cells, hNCs human nasal chondrocytes, HUVECs human umbilical vein endothelial cells, hiPSCs human induced pluripotent stem cells, GelMA gelatin methacrylate, PEGDA polyethylene glycol diacrylate, EY eosin Y, LAP lithium phenyl-2,4,6-trimethyl-benzoyl phosphinate, mBMSCs mouse bone marrow mesenchymal stem cells, NSCs neural stem cells, 3D three-dimensional