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. 2022 Dec 16;23:77. doi: 10.1186/s12868-022-00755-3

Table 1.

Reagents and primers details

Antibodies used for immunocytochemistry Antibody Dilution Cat # and RRID
Pluripotency markers
 Mouse anti-OCT4 1:200 CST Cat.75463
 Rabbit anti-NANOG 1:200 CST Cat.4903 T
 Mouse anti-TRA1-60 1:500 Abcam Cat.ab/6288
 Mouse anti-SSEA4 1:500 CST Cat.MC813
NSC differentiation markers
 Mouse anti-Nestin 1:200 ABcam.Cat.ab18102
 Rabbit anti-sox1 1:200 CST Cat.4194 s
Secondary antibodies
 Mouse anti-IgG 1:1000 CST Cat.4409
 Goat-anti-Rabbit 1:500 Servicebio. Cat.GB25303
Primers Target Forward/Reverse primer (5′-3′)
Targeted mutation ALDH5A1-Exon5 ACGTGACTTTAGCACTAATAAGA AGAGCTTTTAACACTCTGCTGGA
RT-qPCR Primers ALDH5A1-RNA-1 (exons 8–10) GGCACCAGTTATCAAGTTCG ACTCCACCAAAAGGGCACTC
ALDH5A1-RNA-2 (exons 3–4) AGTCATCACCCCGTGGAATTT GAGAAGGGCGTGTCTTCGG
ALDH5A1-RNA-3 (exons 5–6) AGGGGAGGCAATTTGTACTGA GTGGTGCAACAGGATCTTTCC
GAPDH GCACCGTCAAGGCTGAGAAC TGGTGAAGACGCCAGTGG
β-ACTIN CATGTACGTTGCTATCCAGGC CTCCTTAATGTCACGCACGAT
cDNA sequencing F1/R1 TTCCTGTCGCCGTCGTTGC ATCCTACAAGCCCCCGTAACAC
F2-1 GGTCCTCAAGCAGCCCATA
F2-2 TTTGTAAAAGCATTCGCCG
F3 GATGCCGTTTCTAAAGGTGC
R2 AAAATAACCTGCTAACCCAACATC
R3 TTACAAGGACTGGATGAGTTCT