TABLE 1.
Bacterial strains, cell lines, and plasmids used in this study
| Bacterial strain, cell line, or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| S. pyogenes | ||
| NZ131 | M49 | D. R. Martin, Porirua, New Zealand |
| NZ131 speB | speB::ermAM, erythromycin resistant | 5 |
| NZ131 speB emm49 | speB::ermAM; emm49::pRML500; erythromycin and kanamycin resistant | This study |
| 86-858 | M12 | WHO Center for Reference and Research on Streptococci at the University of Minnesota, Minneapolis |
| E. coli DH10B | recA1 | Bethesda Research Laboratories, Inc., Gaithersburg, Md. |
| Cell lines | ||
| CHO-K1 | ATCC CCL 61 | American Type Culture Collection, Manassas, Va. |
| CHO-pgs745 | pgsA-745; neither heparan sulfate nor chondroitan sulfate produced | Jeffrey Esko, University of California—San Diego, La Jolla |
| Plasmids | ||
| pCR2.1 | Cloning vector | Invitrogen |
| pSF141 | Suicide vector; Kanr | 41 |
| pRML500 | 900-bp internal fragment of emm49 cloned into pSF141 | This study |