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. 2022 Dec 15;15:470. doi: 10.1186/s13071-022-05606-y

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Cryptosporidium parvum activated MAPK signaling pathway in HCT-8 cells. HCT-8 cells were stimulated with C. parvum sporozoites from 0 to 24 h, cell lysates were used for western blotting using the rabbit anti-p-ERK, anti-ERK, anti-p-P38 MAPK, anti-P38 MAPK, anti-p-JNK and anti-JNK (A). The results were further analyzed by grayscale analysis from three independent experiments (B, C). A one-way ANOVA assay with Tukey-Kramer post hoc test was used for analyzing data. Data are expressed as the mean ± SD from three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001)