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GAP-43 is downregulated in brn-3b (–/–) E14.5 retina. (A) RT–PCR with primers for brn-3b, GAP-43 and β-actin (as a normalization standard). (B) Staining of E13.5 retinal explants with anti-GAP-43 (green), propidium iodide (red) or neurofilament light chain (blue). +/+ Refers to retinas from wild-type mice and –/– to retinas from brn-3b (–/–) mice.
