a, Anti-FimA immunoblot performed on normalized samples boiled in acidified SDS to depolymerize type 1 pili. b, Anti-FimA immunoblot performed on normalized samples with polymerized type 1 pili. Each band represents FimA polymers of a different size. c, Bacterial hemagglutination titer with or without the FimH inhibitor mannose; mean ± SEM; one-way ANOVA with Dunnett’s test for multiple comparisons. d, Total, adherent, and intracellular bacterial titers in infected urothelial cells; geometric mean ± 95% CI; Kruskal-Wallis test with Dunn’s test for multiple comparisons. All experiments were performed with a minimum of three biological replicates. Each point represents a biological replicate. Exact p-values are provided in the figure, with bold values indicating statistical significance (p < 0.05).