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Restriction maps of the ahpB locus and construction of the ahpA::Kanr cassette, the base of allele exchange. Black boxes represent A. hydrophila AG2 cloned DNA; the thicker black box represents the A. hydrophila ahpB gene, which is oriented from 5′ (left) to 3′ (right). The shaded box represents the Kanr cassette. Horizontal lines represent different plasmid vectors or A. hydrophila ahpA mutant chromosomal DNA.
