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SDS-PAGE and immunoblotting with antibodies to AhpB protease from cell culture supernatants of A. hydrophila AG2 (A), A. hydrophila ahpB mutant (B), and A. salmonicida masoucida containing plasmid pAHE6 (C) in a long-lived experiment. Lanes are culture supernatant samples taken at different hours. Lanes a and b are filtered culture supernatants after incubation 48 and 72 h, respectively, at 37°C. Numbers at the left are molecular masses in kilodaltons.
