FIG. 7.

Proteolytic activity detected on solid media. Macrocolonies of A. hydrophila strains and A. salmonicida masoucida were grown on LB medium supplemented with elastin (A, C4, and C5) or casein (B, C6, and C7) and incubated for 48 h at 28°C. 1, A. hydrophila ahpA mutant; 2, A. hydrophila ahpB mutant; 3, A. hydrophila AG2 wild type; 4 and 6, A. salmonicida masoucida containing plasmid pJRD215; 5 and 7, A. salmonicida masoucida containing plasmid pAHE6. The medium for the later was also supplemented with kanamycin.