Figure 3.

PCR and sequencing amplification of Treponema pallidum by TpN47 were followed by agarose gel electrophoresis to confirm Treponema pallidum sequences for three positive blood and 10 cerebrospinal fluid (CSF) samples. The blood positive control was from one patient’s blood sample (toluidine red unheated serum test [TRUST] titer was 1:256); the CSF positive control was from one patient’s CSF sample (positive serologic assays and metagenomic next-generation sequencing (mNGS); and the negative control was blank without sample.