Figure 5.

Effect of PNAAL on reverse transcription of U5-PBS HIV-1 RNA primed with the labeled 17mer DNA primer. The U5-PBS RNA template primed with the 5′-³²P-labeled 17mer PBS DNA was incubated in the presence or absence of scrambled PNA or increasing concentrations of PNAAL at 37°C. After 2 h incubation, dNTP and HIV-1 RT were supplemented to initiate reverse transcription as described under Materials and Methods. The reaction products were analyzed on a denaturing 8% polyacrylamide–urea gel and subjected to phosphorimager analysis. Lane 1 represents the primer extension in the absence of PNAAL or scrambled PNA. Lane 2 shows the extension of the 17mer primer in the presence of scrambled PNA at a 1:4 molar ratio of template primer to scrambled PNA. Lanes 3–6 depict the primer extension in the presence of PNAAL at 1:1, 1:2, 1:3 and 1:4, molar ratios of template primer to PNAAL, respectively.