Figure 4.

Effect of RBP33 depletion on transcript stability. Actinomycin D and sinefungin were added to control (gray) or RBP33-depleted (black) procyclic trypanosomes. RNA samples were collected at various time points and analyzed by quantitative RT–PCR. Values in each point represent the mean ± SEM of three independent RNAi inductions. Estimated half-life values are indicated (top, RBP33-depleted; bottom, control). Two-sided unpaired Student's t-tests were used to assess whether half-lives were significantly altered upon RBP33 ablation. n.s., not significant (P >0.4); *P <0.05; **P <0.005.