Figure 5.

Effect of exosome depletion on the abundance of cSSR-derived transcripts. (A) Bloodstream and procyclic cell lines were generated that expressed dsRNA corresponding to RRP44 or RRP6 exosome subunits in a tetracycline-inducible manner. Depletion of each protein was monitored by immunoblot after 48 h of tetracycline induction (RNAi). C, control (parental) cell lines. RBP33 served as a loading control in all samples except in procyclic blots, where α-tubulin was used instead. (B and C) Quantitative RT–PCR analysis to measure the levels of transcripts derived from four convergent SSRs. Fold changes (log2 converted) relative to control cells are expressed as the mean (horizontal lines) ± SEM (shaded areas) of three independent RNAi inductions in bloodstream (B) or procyclic (C) trypanosomes.