Table 1.

Concentration of free phosphate liberated by respective protein after incubation with 100 μM pAp detected by Malachite Green phosphate detection assay

	Concentration of phosphate liberated from pAp (mean ± SD) (μM)
Protein	5 min	30 min
B. subtilis NrnA – 5 nM	ND	ND
B. subtilis NrnA – 50 nM	ND	9.7 ± 0.2
B. subtilis NrnA – 500 nM	9.3 ± 0.9	43.7 ± 1.7
E. faecalis NrnA – 5 nM	ND	ND
S. pyogenes Pde2 – 5 nM	ND	ND
M. tuberculosis CnpB – 5 nM	ND	ND
E. coli CysQ – 5 nM	6.3 ± 0.7	31.4 ± 1.2

ND, not detected