TABLE 2.
L. monocytogenes loci identified
| Clonea | Size of ORF productb | Similar proteinsc | Similarityd
|
Functione | |
|---|---|---|---|---|---|
| % Id. | % Sim. | ||||
| Constitutive promoters | |||||
| pt11 | 130 | LaaB; L. sakei | 27 | 54 | Transcriptional regulator |
| pt16 | 140 | LepS; B. subtilis | 46 | 61 | Signal peptidase |
| pt15 | 199 | Maa; E. coli | 65 | 76 | Maltose-acetyltransferase |
| pt24 | 883 | ValS; B. subtilis | 74 | 87 | Valyl-tRNA synthetase |
| pt7 | 220 | YhiD; E. coli | 46 | 61 | Mg2+ ATPase |
| pt19 | 363 | YqgU; B. subtilis | 22 | 41 | Lipoprotein |
| pt32 | 436 | YrvN; B. subtilis | 72 | 81 | Helicase |
| pt31 | 165 | YtgI; B. subtilis | 63 | 76 | Peroxidase |
| pa5 | 338 | LacD; S. aureus | 55 | 75 | Tagatose 1,6-diphosphate aldolase |
| pa308 | 80 | YtmB; B. subtilis | 60 | 76 | Unknown |
| pa4 | 243 | Orf243 | Unknown | ||
| ptS2 | 346 | Asd; B. subtilis | 69 | 82 | Aspartate semialdehyde dehydrogenase |
| ptS8 | 438 | CydA; B. subtilis | 70 | 82 | Cytochrome oxidase |
| ptS1 | 484 | Iap; L. monocytogenes | 100 | 100 | Internalin-associated protein |
| ptS3 | 244 | RluB; B. subtilis | 67 | 85 | Pseudouridine synthetase |
| ptS4 | 431 | SecY; B. licheniformis | 68 | 80 | Preprotein translocase |
| paS8 | 512 | BglI; B. subtilis | 62 | 75 | β-Glucosidase |
| paS3 | 219 | FnR; A. aeolicus | 26 | 44 | Transcriptional regulator (Crp/Fnr family) |
| paB13 | 388 | PatB; B. subtilis | 38 | 62 | Hemolysine |
| paS10 | 640 | ThrS; B. subtilis | 75 | 86 | Threonine-tRNA synthetase |
| In vivo-inducible promoters | |||||
| pa428 | 317 | PlcA; L. monocytogenes | 100 | 100 | PI-PLC |
| pa364 | 373 | YvyH; B. subtilis | 62 | 78 | N-acetyl-glucosamine epimerase |
| pa394 | 104 | Orf104 | 38 | 47 | Lipoprotein |
| pa762 | 591 | PhoR (opposite to hly) | 31 | 52 | Transcriptional regulator |
| pa303 | 301 | MviM (opposite to hly) | 37 | 48 | Virulence factor |
| pa393 | |||||
| pa669 | |||||
| pa769 | |||||
| pa896 | |||||
The clone numbers are preceded by pt for pTCV-derived or pa for pAT28-derived sequences. The pTCV-derived clones (listed alphabetically) are followed by the pAT28-derived clones. Constitutive clones were subdivided in two classes based on whether they were identified as being expressed on blood agar alone (above the line) or as selected in vivo and expressed on blood agar (below the line). A number preceded by a letter means that the clone was screened on blood agar from the spleens (S) or the brains (B) of infected animals.
Size in amino acids of the ORF product encoded by the gene located downstream of the identified promoter.
Name of the ORF product in the databases sharing the highest similarity with the identified ORF product.
Similarity with the identified ORF product. Id., identity; Sim., similarity.
Predicted (on the basis of sequence similarity) or identified function for the ORF product.