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. 2022 Dec 2;9:982399. doi: 10.3389/fmed.2022.982399

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Copyright © 2022 Mizugaki, Wada, Tsuchida, Oda, Kayano, Yamakawa and Tanaka.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of neutrophils and monocytes/macrophages on the dimension reduction map and CD45 expression. (A) Identification of neutrophil islands based on Ly6G and monocyte/macrophage islands based on CD68 in the scatterplot (colored-continuous) of the dimension reduction map, all files concatenated, with the z-channel set to Ly6G or CD68. (B) The expression of CD45 in contour plot, with the files concatenated by groups. CD, cluster of differentiation; CLP, cecal ligation and puncture; Ly6G, lymphocyte antigen 6 G; opt-SNE, optimized parameters for T-distributed stochastic neighbor embedding.