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. 2022 Dec 2;9:982399. doi: 10.3389/fmed.2022.982399

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Copyright © 2022 Mizugaki, Wada, Tsuchida, Oda, Kayano, Yamakawa and Tanaka.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sialic acid-binding lg-like lectin F and Ly6G expression on eosinophils and Siglec-F-positive/negative neutrophils. (A) Identification of eosinophils. (B) Metaclusters re-created with emphasis on Siglec-F expression. MC-I: eosinophils; MC-F and MC-T: Siglec-F-positive neutrophils; MC-C: Siglec-F-negative neutrophils. (C) Histograms of Siglec-F expression. (D) Histograms of Ly6G expression. CD, cluster of differentiation; Ly6G, lymphocyte antigen 6 G; MC, metacluster; opt-SNE, optimized parameters for T-distributed stochastic neighbor embedding; Siglec-F, sialic acid-binding lg-like lectin F.