Table 1. Strains used in this study.
| Name | Description | Genotype |
|---|---|---|
| CA1199 |
Psun-1::TIR1::mRuby
|
unc-119(ed3); ieSi38 [Psun-1::TIR1::mRuby::sun-1 3’UTR, cb-unc-119(+)] IV |
| EU1067 |
GFP::H2B GFP::tubulin |
unc-119(ed3) ruIs32 [unc-119(+) pie-1::GFP::H2B] III; ruIs57 [unc-119(+) pie-1::GFP::tubulin] |
| EU2861 | GFP::aspm-1 | or1935 [GFP::aspm-1] I |
| EU2876 |
GFP::aspm-1 mCherry::H2B |
or1935 [GFP::aspm-1] I; ltIs37 [pAA64; pie-1::mCherry::his-58; unc-119(+)] IV |
| JA1559 | mCherry::tubulin | weIs21 [pJA138 (pie-1::mCherry::tub::pie-1)]; unc-119(ed3) III |
| OD56 | mCherry::H2B | ltIs37 [(pAA64) pie-1::mCherry::his-58 + unc-119(+)] IV |
| OD57 |
mCherry::H2B GFP::tubulin |
unc-119(ed3) III; ltIs37 [pAA64; pie-1::mCherry::his-58; unc-119(+)] IV; ltIs25 [pAZ132; pie-1::GFP::tba-2; unc-119 (+)] |
| PHX3422 |
GFP::degron::tac-1 Psun-1::TIR1::mRuby |
tac-1(syb3422) II; ieSi38 IV |
| SMW21 |
Psun-1::TIR1::mRuby mCherry::H2B |
unc-119(ed3); ieSi38 [Psun-1::TIR1::mRuby::sun-1 3’UTR, cb-unc-119(+)] IV; ltIs37 [(pAA64) pie-1::mCherry::his-58 + unc-119(+)] IV |
| SMW22 |
Psun-1::TIR1::mRuby mCherry::tubulin |
unc-119(ed3) III; weIs21 [pJA138 (pie-1::mCherry::tubulin::pie-1)]; ieSi38 IV |
| SMW24 |
degron::GFP::zyg-9 Psun-1::TIR1::mRuby |
Pzyg-9::degron::EmGFP::zyg-9 (C6->T—PAM site mutation) II; unc-119(ed3); ieSi38 [Psun-1::TIR1::mRuby::sun-1 3’UTR, cb-unc-119(+)] IV |
| SMW26 |
degron::GFP::zyg-9 mCherry::H2B Psun-1::TIR1::mRuby |
Pzyg-9::degron::EmGFP::zyg-9 (C6->T—PAM site mutation) II; unc-119(ed3); ieSi38 [Psun-1::TIR1::mRuby::sun-1 3’UTR, cb-unc-119(+)] IV; ltIs37 [(pAA64) pie-1::mCherry::his-58 + unc-119(+)] IV |
| SMW31 |
Psun-1::TIR1::mRuby GFP::H2B GFP::tubulin |
unc-119(ed3) III; ruIs32[unc-119(+) pie-1::gfp::h2b] III; ruIs57[unc-119(+) pie-1::GFP::tubulin]; ieSi38 IV |
| SMW33 |
degron::GFP::zyg-9 mCherry::tubulin Psun-1::TIR1::mRuby |
Pzyg-9::degron::EmGFP::zyg-9 (C6->T—PAM site mutation) II; unc-119(ed3); ieSi38 [Psun-1::TIR1::mRuby::sun-1 3’UTR, cb-unc-119(+)] IV; weIs21 [pJA138 (pie-1::mCherry::tub::pie-1)]; unc-119(ed3) III |
| SMW71 |
GFP::aspm-1 degron::GFP::zyg-9 mCherry::tubulin Psun-1::TIR1::mRuby |
or1935 [GFP::aspm-1] I; Pzyg-9::degron::EmGFP::zyg-9 (C6->T—PAM site mutation) II; ieSi38 [Psun-1::TIR1::mRuby::sun-1 3’UTR, cb-unc-119(+)] IV; weIs21 [pJA138 (pie-1::mCherry::tub::pie-1)]; unc-119(ed3) III |