|
dpy-10 crRNA |
5’-GCUACCAUAGGCACCACGAG-3’ |
|
dpy-10 repair oligo (Ultramer from IDT) |
5’-CACTTGAACTTCAATACGGCAAGATGAGAAT GACTGGAAACCGTACCGCATGCGGTGCCTATG GTAGCGGAGCTTCACATGGCTTCAGACCAACAGCCTAT-3’ |
|
zyg-9 crRNA |
5’-CUCGUCCAGAUAAUCCCAAU-3’ |
|
zyg-9 repair-F1 |
5’-ACGTAGTAAACTGTCATTTTTCAGATAATGCCTAAA GATCCAGCCAAACC-3’ |
|
zyg-9 repair-R1 |
5’-CCACCTCGTCCAGATAATCCCAATTAGACATTCTAG AGCGGCCGCCA-3’ |
|
zyg-9 repair-F2 |
5’-TTCGTTCGCTTTCTTTGTTTATTGCAAGGCACGTA GTAAACTGTCATTTTTCAG-3’ |
|
zyg-9 repair-R2 |
5’-CGAAGTTCGGTGGAAGTTTGGGAAGGATATCCACC TCGTCCAGATAATC-3’ |
|
zyg-9 insertion check-F |
5’-CGGAAATCTATTGTTGAAATCTCCTTTC-3’ |
|
zyg-9 insertion check-R |
5’-CTTTCATTTTTTCGAAAATGACGGG-3’ |
| pADR28 |
degron::EmGFP::linker plasmid template for PCR reactions to generate zyg-9 repair template. Derived from pLZ29. |
