Table 4.
Main reagents.
| Name | Manufacturer |
|---|---|
| PolyATtract System 1000 mRNA extraction kit | Promega |
| THERMOScript™ RT-PCR System | Gibco/BRL |
| ZAP-cDNA systhesis kit | Stratagene |
| ZAP-cDNA Gigapack III Gold Cloning kit | Stratagene |
| α-32p-dATP, α-32p-dCTP 3000 ci/mM | Beijing Yahui Company |
| CNBr-Sepharose 4B | Amersham |
| IPTG | Merk |
| Nitrocellulose membrane | Pall |
| Goat anti-human IgG-AP | Sigma |
| NBT/BCIP | Sigma |
| EcoRI | New England Biotech |
| XhoI | New England Biotech |
| DNA small amount extraction kit | Promega |
| DNA recovery kit | BioDev-Tech. Co., Ltd |
| EcoRI | NEB |
| XhoI | NEB |
| IPTG | Merk |
| Ni-NTA His-Bind Resins | Novagen |
| Mouse anti-(His) antibody | Pharmacia |
| Goat anti-mouse IgG-HRP | Sigma |
| M-MLV | Invitrogen |
| Oligo(dT)15 | Promega |
| dNTP | Promega |
| RNasin | Promega |
| Taq enzyme | Promega |
| xMAP® Antibody Coupling Kit | Luminex |
| Magplex® Microspheres | Luminex |
| R-Phycoerythrin AffiniPure F(ab’)2 Fragment Donkey Anti-Human IgG (H+L) | Jackson ImmunoResearch |